Publication: Identification of proliferating neural progenitors in the adult human hippocampus.

Pubmed ID: 40608919

Journal: Science (New York, N.Y.)

Publication Date: July 3, 2025

Affiliation: Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.

MeSH Terms: RNA-Seq, Ki-67 Antigen, Machine Learning, Dentate Gyrus, Infant, Infant, Newborn, Neurogenesis, Cell Proliferation, Hippocampus, Neural Stem Cells, Sequence Analysis, RNA, Child, Preschool, Child, Single-Cell Analysis, Aged, Young Adult, Middle Aged, Transcriptome, Adult, Adolescent, Male, Female, Humans

Authors: Tata M, Dumitru I, Zamboni M, Saghaleyni R, Giatrellis S, Alkass K, Björklund Å, Druid H, Sandberg R, Frisén J, Paterlini M, Ziegenhain C

Cite As: Dumitru I, Paterlini M, Zamboni M, Ziegenhain C, Giatrellis S, Saghaleyni R, Björklund Å, Alkass K, Tata M, Druid H, Sandberg R, Frisén J. Identification of proliferating neural progenitors in the adult human hippocampus. Science (New York, N.Y.). 2025 Jul 3.

Abstract

Continuous adult hippocampal neurogenesis is involved in memory formation and mood regulation but is challenging to study in humans. Difficulties finding proliferating progenitor cells called into question whether and how new neurons may be generated. We analyzed the human hippocampus from birth through adulthood by single-nucleus RNA sequencing. We identified all neural progenitor cell stages in early childhood. In adults, using antibodies against the proliferation marker Ki67 and machine learning algorithms, we found proliferating neural progenitor cells. Furthermore, transcriptomic data showed that neural progenitors were localized within the dentate gyrus. The results contribute to understanding neurogenesis in adult humans.